IFN-gamma ELISA Kit Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is advised for the quantitative altitude of IFN gamma in supernatants, buffered solutions or serum and claret samples and added physique fluids.
A monoclonal antibiotic specific for IFN gamma has been coated assimilate the wells of the microtiter strips provided. Samples, including standards of accepted IFN gamma concentrations, ascendancy specimens or unknowns are pipetted into these wells. During the aboriginal incubation, the standards or samples and a biotinylated monoclonal antibiotic specific for IFN gamma are accompanying incubated. After washing, the agitator Streptavidin-HRP, that binds the biotinylated antibiotic is added, incubated and washed. A TMB substrate band-aid is added which acts on the apprenticed agitator to abet a black acknowledgment product. The acuteness of this black artefact is anon proportional to the absorption of IFN gamma present in the samples.
This kit will admit both autogenous and recombinant Human IFN gamma.